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STAT3 plays an important role in DNA replication by turning on WDHD1

Yunying Zhou1,2,3, Jason J Chen4,5

  • 1Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Cell & Bioscience
|January 8, 2021
PubMed
Abstract

Insights

Signal transducers and activators of transcription 3 (STAT3) regulates DNA replication. STAT3 targets the WD repeat and HMG-box DNA-binding protein 1 (WDHD1) gene, revealing a novel role in cell proliferation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Signal transducers and activators of transcription 3 (STAT3) is a transcription factor involved in cell growth and cancer.
  • The precise mechanisms of STAT3 in regulating cellular processes remain incompletely understood.

Purpose of the Study:

  • To elucidate novel functions and regulatory mechanisms of STAT3.
  • To investigate the role of STAT3 in DNA replication.

Main Methods:

  • Gene knockdown and ectopic expression of STAT3.
  • Analysis of DNA replication rates.
  • Identification and validation of STAT3 target genes using promoter binding assays.

Main Results:

  • STAT3 plays a crucial role in DNA replication; its knockdown inhibited replication, while activation promoted it.
  • WD repeat and HMG-box DNA-binding protein 1 (WDHD1), a key regulator of DNA replication initiation, was identified as a novel STAT3 target gene.
  • STAT3 was shown to bind the promoter/upregulatory region of the WDHD1 gene.

Conclusions:

  • A novel function of STAT3 in regulating DNA replication has been identified.
  • This function is mediated through its newly identified target gene, WDHD1.
  • These findings have significant implications for understanding cell growth and cancer biology.
Keywords:
DNA replicationSTAT3TranscriptionWDHD1

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