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Complete Genome Sequence of Streptomyces Phage Spernnie

Nathaniel Tate1, Loraine Melbern1, James Clark2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|January 8, 2021
PubMed

Insights

We characterized Spernnie, a temperate siphophage infecting Streptomyces bacteria. This phage has a 50,834-bp genome with 89 predicted coding genes, offering insights into Streptomyces phage biology.

Area of Science:

  • Microbiology
  • Bacteriology
  • Virology

Background:

  • Streptomyces species are Gram-positive soil bacteria.
  • Some Streptomyces strains can cause serious human infections like mycetomas, pneumonia, and septicemia.

Purpose of the Study:

  • To characterize a novel temperate siphophage, named Spernnie.
  • To analyze the genome of Spernnie infecting Streptomyces sp. strain Mg1.

Main Methods:

  • Genome sequencing of Spernnie.
  • Bioinformatic analysis of the phage genome to predict coding genes.

Main Results:

  • Spernnie is a temperate siphophage with a genome size of 50,834 base pairs.
  • The Spernnie genome contains 89 predicted coding genes.

Conclusions:

  • The characterization of Spernnie provides new information on the genetic makeup of phages infecting Streptomyces.
  • Understanding these phages can contribute to the study of Streptomyces pathogenesis and phage therapy development.

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