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Updated: Nov 18, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Single-cell RNA Sequencing in Immunology.

Yirui Cao¹, Yue Qiu¹, Guowei Tu¹

¹1Department of Urology, Zhongshan Hospital, Fudan University; Shanghai Key Laboratory of Organ Transplantation, Shanghai200032, China; 2Department of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai200032, China; 3Fudan Zhangjiang Institute of Fudan University, Shanghai201203, China.

Current Genomics

|January 8, 2021

Summary

Single-cell RNA sequencing (scRNA-seq) analyzes immune cells in diseases. This review overviews scRNA-seq applications in immunology and future research directions.

Keywords:

Single-cell RNA sequencing autoimmune disease cancer immunology transplantation tumors

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Last Updated: Nov 18, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

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Published on: January 7, 2020

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

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Area of Science:

Immunology
Genomics
Molecular Biology

Background:

The immune system's complexity underlies many diseases.
Single-cell RNA sequencing (scRNA-seq) offers high-resolution analysis of cellular components.
Understanding immune responses is crucial for disease pathology.

Purpose of the Study:

To review the applications of scRNA-seq in immunology.
To highlight innovative findings from scRNA-seq studies in disease.
To prospect future directions for scRNA-seq in immunological research.

Main Methods:

Literature review of scRNA-seq studies in immunology.
Analysis of scRNA-seq data for immune cell profiling.
Synthesis of current research trends and future potential.

Main Results:

scRNA-seq enables detailed analysis of immune cell heterogeneity.
Numerous studies have utilized scRNA-seq to uncover disease-specific immune mechanisms.
Innovative results demonstrate the power of scRNA-seq in dissecting complex immune responses.

Conclusions:

scRNA-seq is a powerful tool for advancing immunological research.
Its application is expanding across various disease areas.
Future directions include refining methodologies and broadening applications.