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Updated: Nov 22, 2025
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Published on: January 6, 2015
SoluProt: prediction of soluble protein expression in Escherichia coli
Jiri Hon1,2,3, Martin Marusiak3, Tomas Martinek3
1Loschmidt Laboratories, Centre for Toxic Compounds in the Environment RECETOX and Department of Experimental Biology, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic.
Motivation:
Poor protein solubility hinders the production of many therapeutic and industrially useful proteins. Experimental efforts to increase solubility are plagued by low success rates and often reduce biological activity. Computational prediction of protein expressibility and solubility in Escherichia coli using only sequence information could reduce the cost of experimental studies by enabling prioritization of highly soluble proteins.
Results:
A new tool for sequence-based prediction of soluble protein expression in E.coli, SoluProt, was created using the gradient boosting machine technique with the TargetTrack database as a training set. When evaluated against a balanced independent test set derived from the NESG database, SoluProt's accuracy of 58.5% and AUC of 0.62 exceeded those of a suite of alternative solubility prediction tools. There is also evidence that it could significantly increase the success rate of experimental protein studies. SoluProt is freely available as a standalone program and a user-friendly webserver at https://loschmidt.chemi.muni.cz/soluprot/.
Availability And Implementation:
https://loschmidt.chemi.muni.cz/soluprot/.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
