Significantly improving the quality of genome assemblies through curation
Kerstin Howe1, William Chow1, Joanna Collins1
1Tree of Life, Wellcome Sanger Institute, Cambridge CB10 1SA, UK.
Gigascience
|January 9, 2021
Summary
Generating accurate genome assemblies remains challenging for eukaryotes. This study presents gEVAL, a genome evaluation browser, to improve assembly quality through curation, enhancing biological understanding.
Area of Science:
- Genomics
- Bioinformatics
Background:
- High-quality genome assemblies are fundamental for biological research.
- Automated genome assembly methods still struggle to produce error-free eukaryotic genomes.
Purpose of the Study:
- To present and recommend a genome assembly curation approach using the gEVAL tool.
- To facilitate wider adoption of genome curation practices.
Main Methods:
- Utilizing the genome evaluation browser (gEVAL) for assembly curation.
- Describing specific procedures for genome curation with gEVAL.
- Providing recommendations for gEVAL-independent genome curation.
Main Results:
- Assembly curation significantly reduces errors in genome sequences.
- Insights from curation improve automated assembly strategies.
- gEVAL facilitates effective genome evaluation and error correction.
Conclusions:
- Genome assembly curation is essential for achieving high-quality eukaryotic genomes.
- The gEVAL tool and recommended practices enhance genome assembly accuracy.
- Curation feedback loops contribute to advancing automated assembly pipelines.
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