Related Experiment Video
Updated: Nov 22, 2025
Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
Published on: July 7, 2023
Bisphenol S is present in culture media used for ART and cell culture
A Togola1, A Desmarchais2, O Téteau2
1BRGM, Orléans Cedex 245060, France.
Study Question:
Do plastic laboratory consumables and cell culture media used in ART contain bisphenols?
Summary Answer:
The majority of human embryo culture media assessed contained bisphenol S close to the nanomolar concentration range, while no release of bisphenols by plastic consumables was detected under routine conditions.
What Is Known Already:
The deleterious effect of the endocrine disruptor bisphenol A (BPA) on female fertility raised concerns regarding ART outcome. BPA was detected neither in media nor in the majority of plastic consumables used in ART; however, it might have already been replaced by its structural analogs, including bisphenol S (BPS).
Study Design, Size, Duration:
Seventeen plastic consumables and 18 cell culture and ART media were assessed for the presence of bisphenols.
Participants/Materials, Setting, Methods:
Ten different bisphenols (bisphenol A, S, AF, AP, B, C, E, F, P and Z) were measured using an isotopic dilution according to an on-line solid phase extraction/liquid chromatography/mass spectrometry method.
Main Results And The Role Of Chance:
While the plastic consumables did not release bisphenols under routine conditions, 16 of the 18 cell culture and ART media assessed contained BPS. Six media exhibited BPS concentrations higher than 1 nM and reached up to 6.7 nM (1693 ng/l).
Large Scale Data:
N/A.
Limitations, Reasons For Caution:
Further studies are required to investigate a greater number of ART media to identify less potentially harmful ones, in terms of bisphenol content.
Wider Implications Of The Findings:
As BPS has already been reported to impair oocyte quality at nanomolar concentrations, its presence in ART media, at a similar concentration range, could contribute to a decrease in the ART success rate. Thus far, there has been no regulation of these compounds in the ART context.
Study Funding/Competing Interests:
This study was financially supported by the 'Centre-Val de Loire' Region (Bemol project, APR IR 2017), INRAE, BRGM, the French National Research Agency (project ANR-18-CE34-0011-01 MAMBO) and the BioMedicine Agency (Project 18AMP006 FertiPhenol). The authors declare that they have no conflict of interest that could be perceived as prejudicing the impartiality of the reported research.
Related Concept Videos
01:21Cell Culture
01:16Cell Lines