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Immunomodulation Through Beta-D-glucan in Chemically-induced Necrotizing Pancreatitis
Nikolaos N Koliakos1, Georgios Renieris2, Dimitrios Sotiropoulos1
13rd Department of Surgery, National and Kapodistrian University of Athens, Medical School, Athens, Greece.
The Journal of Surgical Research
|January 9, 2021
Summary
Beta-D-glucan significantly improved survival in a rabbit model of necrotizing pancreatitis. This immune modulator reduced inflammation and bacterial translocation, offering a potential therapeutic strategy for this severe condition.
Area of Science:
- Immunology
- Gastroenterology
- Sepsis Research
Background:
- The immunomodulatory effects of beta-D-glucan and monophosphoryl lipid A (MPLA) are known in human cells.
- Their impact on sterile inflammation, specifically necrotizing pancreatitis, remains uninvestigated.
Purpose of the Study:
- To investigate the efficacy of beta-D-glucan and MPLA in a rabbit model of necrotizing pancreatitis.
- To assess their effects on survival, pancreatic inflammation, and bacterial load.
Main Methods:
- 85 rabbits underwent sodium taurocholate-induced pancreatitis.
- Groups received beta-D-glucan or MPLA pre- or post-induction.
- Survival, amylase levels, cytokine production (TNF-α, IL-10), pancreatic necrosis, and bacterial load were evaluated.
Main Results:
- Beta-D-glucan administration significantly prolonged 21-day survival.
- Both beta-D-glucan and MPLA reduced pancreatic necrosis and tissue bacterial load.
- Beta-D-glucan treatment decreased TNF-α production while IL-10 remained unchanged.
Conclusions:
- Beta-D-glucan demonstrates significant mortality reduction in experimental pancreatitis.
- Its therapeutic benefits are linked to reduced cytokine production and prevention of bacterial translocation.
