Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment

Fluorescence imaging provides a powerful technique to observe biomolecular dynamics in living systems, if fluorescent biosensors for the relevant biomolecules become available. Here, we describe a highly sensitive, cell-based biosensor to visualize nitric oxide (NO) released from living cells. Nitric oxide (NO) is a gaseous molecule that is involved in a broad range of physiological and toxicological processes in cardiovascular and central nervous systems, etc. This chapter describes how to make optical measurements of NO release from living cells using the cell-based fluorescent biosensor.