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Updated: Nov 22, 2025
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
Published on: January 7, 2019
Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function, and Cell Fate Along
Mariana Dutra Brito1, Luiz Felipe Souza E Silva1, Amanda Siena1
1Department of Physiological Science, Santa Casa de São Paulo School of Medical Science. R. Doutor Cesário Motta Júnior, São Paulo, SP, Brazil.
Abstract:
Depletion of oxygen (O2) levels and reduction in the ATP synthesis (or even its complete blockage) are important characteristics of mitochondrial dysfunction; features that are often correlated with neurodegeneration. The measurement of oxygen consumption rate (OCR) is thus essential to evaluate cellular metabolism, survival, and neuroprotective strategies. In the present chapter, we describe the oxygen consumption assay using a Clark-type oxygen electrode in different types of samples named cells suspension (from primary and established cell culture), brain slices (ex vivo), and fresh brain tissues. In addition, we demonstrate herein how the program Oxygraph can be used in order to analyze the data and different approaches to normalize it.
