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Updated: Nov 22, 2025

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
Published on: September 26, 2019
Characterisation of black skin stratum corneum by digital macroscopic images analysis
Géraud M Azehoun-Pazou1,2, Kokou M Assogba2, Hugues Adegbidi3
1National University of Sciences, Technologies, Engineering and Mathematics (UNSTIM), BP 2282 Abomey, Benin.
Abstract:
Black skin medical images generally show very low contrast. Being in a global initiative of characterisation of black skin horny layer (stratum corneum) by digital images analysis, the authors in this study proposed a four-step approach. The first step consists of differentiation between probable healthy skin regions and those affected. For that, they used an automatic classification system based on multilayer perceptron artificial neural networks. The network has been trained with texture and colour features. Best features selection and network architecture definition were done using sequential network construction algorithm-based method. After classification, selected regions undergo a colour transformation, in order to increase the contrast with the lesion region. Thirdly, created colour information serves as the basis for a modified fuzzy c-mean clustering algorithm to perform segmentation. The proposed method, named neural network-based fuzzy clustering, was applied to many black skin lesion images and they obtained segmentation rates up to 94.67%. The last stage consists in calculating characteristics. Eight parameters are concerned: uniformity, standard deviation, skewness, kurtosis, smoothness, entropy, and average pixel values calculated for red and blue colour channels. All developed methods were tested with a database of 600 images and obtained results were discussed and compared with similar works.
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