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Updated: Nov 22, 2025

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe
Joydev Hatai1, Pragati Kishore Prasad, Naama Lahav-Mankovski
1Department of Organic Chemistry, Weizmann Institute of Science, Rehovot 7610001, Israel. leila.motiei@weizmann.ac.il david.margulies@weizmann.ac.il.
Abstract:
Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.
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