Highly selective enrichment and direct determination of imazethapyr residues from milk using magnetic solid-phase
Shicong Jia1, Yanqiang Zhou, Jianmin Li
1School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan, P. R. China. gongbolin@163.com.
Abstract:
Restricted access media magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared as magnetic solid phase extraction (M-SPE) material by reversible addition fragmentation chain transfer (RAFT) technique. The resulting RAM-MMIPs had a uniform, imprinted, hydrophilic layer (63 nm), good binding capacity (34.85 mg g-1) and satisfactory selectivity. In addition, these RAM-MMIPs had a robust ability to eliminate the interference of protein macromolecules. These RAM-MMIPs were then coupled with HPLC/UV to identify imazethapyr (IM) residues in untreated milk samples. Several major factors would affect M-SPE extraction efficiency, such as the amount of RAM-MMIPs, pH, extraction time of the sample solution, and the volume ratio of the elution solvent. Under the optimal conditions, the developed method had good linearity (R2 > 0.9993), low detection limit (2.13 μg L-1), and low quantitative limit (7.15 μg L-1). These results indicated this proposed approach is an efficient method for direct enrichment and detection of IM herbicides in milk and other biological samples.
