Cucurbituril and zwitterionic surfactant-based matrix solid-phase dispersion microextraction to simultaneously...

Area of Science:

• Analytical Chemistry
• Natural Product Chemistry
• Pharmacognosy

Background:

• Radix Curcumae is a traditional medicine with valuable terpenoid compounds.
• Accurate quantification of these terpenoids is crucial for quality control and pharmacological research.
• Existing extraction methods may lack efficiency, environmental friendliness, or sensitivity.

Purpose of the Study:

• To develop and validate a rapid, efficient, and environmentally friendly method for terpenoid determination in Radix Curcumae.
• To optimize extraction parameters using single-factor and response surface methodology.
• To apply the validated method for the analysis of specific terpenoids in Radix Curcumae samples.

Main Methods:

• Matrix solid-phase dispersion microextraction (MSPD) coupled with ultra-high-performance liquid chromatography (UHPLC) and a diode array detector (DAD).
• Optimization of grinding time, adsorbent amount, elution solvent type/concentration, and pH.
• Validation of the method for linearity, recovery, and reproducibility.

Main Results:

• Optimized conditions: 70 mg cucurbituril, 149 s grinding, 228 mM 3-(N,N-dimethylpalmitylammonio)propanesulfonate (pH 6.5) elution solvent.
• Satisfactory linearity (R² 0.9945–0.9970) and high recoveries (96.53–104.60%) for five key terpenoids.
• Acceptable reproducibility with relative standard deviation ≤ 3.66%.

Conclusions:

• The developed MSPD-UHPLC method is rapid, efficient, and environmentally friendly for terpenoid analysis in Radix Curcumae.
• The method provides accurate and reliable quantification of curdione, isocurcumenol, furanodienone, germacrone, and furanodiene.
• This approach offers a valuable tool for the quality assessment and research of Radix Curcumae.