Modified improvised pre-embedding method for core needle biopsies: A clinicopathologic study

Area of Science:

• Surgical Pathology
• Histotechnology

Background:

• Core needle biopsy (CNB) is crucial for tissue diagnosis and treatment selection.
• Current CNB processing lacks standardized guidelines, leading to variable laboratory practices.
• A user-friendly, cost-effective pre-embedding method is needed to ensure intact CNB cores.

Purpose of the Study:

• To compare the diagnostic outcomes of CNBs processed via a conventional method versus a modified pre-embedded protocol.
• To evaluate the effectiveness of a novel pre-embedding technique for CNB specimens over a two-year period.

Main Methods:

• CNBs from solid organ lesions (SOL) were processed using either the conventional free-floating formalin method (control) or a modified pre-embedding technique with polyurethane foam meshes and coloring inks (test).
• Specimens were assessed for core intactness, tissue processing quality, ease of embedding, and ease of section cutting.
• Statistical analysis was performed on the collected data.

Main Results:

• CNBs processed with the modified pre-embedding method demonstrated superior intactness, linearity, and alignment compared to the control group.
• The number of blocks and sections required was reduced by 25% when processing four CNBs within a single block using the new method.
• The modified technique proved more economical and efficient.

Conclusions:

• The pre-embedding method utilizing polyurethane foam and coloring inks offers superior results compared to conventional free-floating CNB processing.
• This technique is economical, user-friendly, and can be implemented at the bedside by surgeons.
• The improved processing enhances diagnostic yield and efficiency in histopathology.