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Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
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Related Experiment Video

Updated: Nov 21, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Precise electroosmotic flow measurements on paper substrates.

Nicolás Franck¹, Federico Schaumburg², Pablo A Kler^1,3

¹Centro de Investigación en Métodos Computacionales (CIMEC, UNL-CONICET), Colectora RN 168 Km 472, Santa Fe, S3000GLN, Argentina.

Electrophoresis

|January 12, 2021

Summary

This summary is machine-generated.

A new method accurately measures electroosmotic flow (EOF) in paper-based analytical devices using an analytical balance. This technique provides crucial data for selecting paper substrates for microfluidic applications.

Keywords:

Electroosmosis Electrophoresis Paper-based microfluidics Whatman #1

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Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

Area of Science:

Analytical Chemistry
Microfluidics
Materials Science

Background:

Microfluidic paper-based analytical devices (μPADs) are increasingly used for diagnostics.
Accurate characterization of fluid flow, specifically electroosmotic flow (EOF), is crucial for μPAD performance.
Existing EOF measurement methods for porous media like paper can be unreliable or complex.

Purpose of the Study:

To present a novel, reliable, and simple method for measuring EOF on paper substrates.
To provide enhanced information on physical parameters governing fluid flow in μPADs.
To establish electroosmotic characteristics of common filter papers for informed substrate selection.

Main Methods:

Developed a method based on dynamic mass measurements using an analytical balance.
Performed measurements on Whatman #1, S&S, and Muntktell 00A filter papers.
Quantified influencing factors such as Joule heating and evaporation.

Main Results:

The novel method provides reliable EOF measurements on paper substrates.
Experimental results align with existing literature data for EOF in paper.
Established the relationship between EOF magnitude and pH for different paper types.
Quantified the impact of Joule effect and evaporation on measurements.

Conclusions:

The presented method is robust, reliable, and affordable for EOF measurement in μPADs.
Enables informed selection of paper substrates based on specific electrophoretic requirements.
Advances the understanding and development of paper-based microfluidic devices.