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Self-Activated Fluorescent Hydroxyapatite Nanoparticles: A Promising Agent for Bioimaging and Biolabeling
Ketaki Deshmukh1, M Monsoor Shaik2, Sutapa Roy Ramanan2
1Biological Sciences Department and ‡Chemical Engineering Department, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Zuarinagar, Goa 403726, India.
ACS Biomaterials Science & Engineering
|January 13, 2021
Summary
Researchers developed novel, nontoxic fluorescent hydroxyapatite nanoparticles (fHAps) for bioimaging. These self-activated nanoparticles offer a safe and effective alternative for advanced medical diagnostics and biological visualization.
Area of Science:
- Biomedical Engineering
- Materials Science
- Nanotechnology
Background:
- Bioimaging advancements have significantly improved medical diagnostics and biological process visualization.
- Fluorescence imaging with nontoxic nanomaterials offers high sensitivity, minimal invasiveness, and safety.
Purpose of the Study:
- To synthesize and characterize novel, self-activated fluorescent hydroxyapatite nanoparticles (fHAps).
- To evaluate the potential of fHAps as safe alternatives for biolabeling and bioimaging.
Main Methods:
- Synthesized fHAps with varying aspect ratios using different stabilizing agents and solvents.
- Characterized fHAps using fluorescence spectroscopy and Electron Spin Resonance (ESR) spectroscopy.
- Assessed nanoparticle toxicity and cellular internalization in prokaryotic and eukaryotic systems.
Main Results:
- Achieved excellent fluorescence emission from 350 to 750 nm (peak at 502 nm) in fHAps.
- Confirmed fluorescence origin from electronic transitions in asymmetric fHAp structures via ESR.
- Demonstrated nontoxicity and efficient internalization of fHAps in various biological systems.
Conclusions:
- Nontoxic, self-activated fHAps exhibit promising fluorescence properties for bioimaging.
- fHAps present a safe and effective alternative to existing fluorescent materials for biolabeling.
- The unique properties of fHAps open new avenues in medical diagnostics and biological research.
