Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

4.1K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.1K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.4K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.4K
Telomeres and Telomerase02:41

Telomeres and Telomerase

25.9K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
25.9K
Telomeres and Telomerase02:41

Telomeres and Telomerase

6.4K
6.4K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

4.0K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
4.0K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.6K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ROR1/ROR2 regulate aldosterone production from the zona glomerulosa.

Endocrinology·2026
Same author

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026
Same author

Loss of WNT2B Increases Tumor Burden and Malignant Features in Colorectal Cancer.

Cancer communications (London, England)·2026
Same author

EGFR INHIBITION PROMOTES ENTEROENDOCRINE CELL DIFFERENTIATION CONTRIBUTING TO TREATMENT-ASSOCIATED DIARRHEA.

bioRxiv : the preprint server for biology·2026
Same author

Early-life mucosal T cells direct intestinal stem cell fate via a coordinated developmental program.

bioRxiv : the preprint server for biology·2026
Same author

Peroxisome proliferator-activated receptor-α activation attenuates susceptibility to postoperative atrial fibrillation.

Journal of thoracic disease·2026

Related Experiment Video

Updated: Nov 21, 2025

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
09:44

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

3.3K

Telomerase expression marks transitional growth-associated skeletal progenitor/stem cells.

Diana L Carlone1,2,3, Rebecca D Riba-Wolman1,2, Luke T Deary1

  • 1Division of Endocrinology, Boston Children's Hospital, Boston, Massachusetts, USA.

Stem Cells (Dayton, Ohio)
|January 13, 2021
PubMed
Summary

Telomerase (Tert) expression marks specific skeletal stem cells (SSCs) during adolescent bone growth. These mTert-expressing SSCs contribute to bone formation and maintenance, highlighting a critical role in skeletal development.

Keywords:
mTertpluripotentpostnatal bone growthskeletal stem cellstelomerase

More Related Videos

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

2.2K
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
08:34

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

10.6K

Related Experiment Videos

Last Updated: Nov 21, 2025

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
09:44

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

3.3K
Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

2.2K
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
08:34

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

10.6K

Area of Science:

  • Skeletal Biology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Skeletal progenitor/stem cells (SSCs) are crucial for postnatal bone growth and repair.
  • Telomerase (Tert) activity is vital for maintaining stem cell function and preventing senescence in self-renewing tissues.

Purpose of the Study:

  • To investigate the role and characteristics of mTert-expressing cells in postnatal mouse long bone development.
  • To identify the contribution of mTert-expressing cells to skeletal lineages during growth and maintenance.

Main Methods:

  • Utilized mTert-GFP reporter mice to track mTert expression.
  • Identified mTert-GFP+ cells in key SSC niches within the long bone.
  • Performed in vitro colony-forming assays and in vivo lineage tracing studies.

Main Results:

  • mTert expression is enriched during adolescent bone growth in mouse long bones.
  • mTert-expressing cells are located in SSC-rich regions: metaphyseal stroma, growth plate, and bone marrow.
  • mTert+ cells exhibit enhanced colony-forming capacity in vitro and function as osteochondral progenitors in vivo, contributing to endochondral bone formation.

Conclusions:

  • mTert expression is temporally regulated, identifying a distinct population of skeletal stem cells during transitional growth.
  • These mTert-expressing cells are critical osteochondral progenitors during endochondral ossification and persist into adulthood.