Biocidal and Antifouling Chlorinated Protein Films.

Area of Science:

• Biomaterials Science
• Surface Chemistry
• Microbiology

Background:

• Bacterial adhesion to medical devices causes severe infections.
• Nonfouling and biocidal coatings are crucial for preventing device-related infections.
• Combining these properties offers superior antimicrobial efficacy.

Purpose of the Study:

• To develop a novel antibacterial coating using nanoimprint lithography (NIL).
• To create a coating that resists bacterial attachment and exhibits biocidal activity.
• To provide a new strategy for preventing infections associated with medical devices and implants.

Main Methods:

• Utilized nanoimprint lithography (NIL) to create water-stable bovine serum albumin (BSA) films.
• Engineered films with negative/zwitterionic surface potential to achieve non-adhesiveness.
• Incorporated biocidal properties via chlorination of cysteine sulfurs for slow chlorine release.

Main Results:

• Successfully generated antibacterial coatings with dual nonfouling and biocidal properties.
• BSA films demonstrated resistance to bacterial attachment due to surface charge.
• Chlorinated BSA films exhibited potent antimicrobial activity against pathogenic bacteria.

Conclusions:

• NIL is an effective method for creating advanced antibacterial coatings.
• The developed BSA-based coatings offer a promising solution for preventing medical device-related infections.
• This approach combines nonfouling and biocidal functionalities for enhanced antimicrobial performance.