Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.6K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.6K
Synthetic Biology02:55

Synthetic Biology

5.2K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular versatility of polyproline II helices: from natural proteins to biomimetic materials.

Journal of materials chemistry. B·2026
Same author

Understanding Biases in Liquid-Liquid Phase Separation: Investigating Amino Acid Enrichments in Phase-Separating Proteins toward Peptide Design.

Biomacromolecules·2025
Same author

Fast and flu-rious: How to prevent and treat emerging influenza A viruses.

PLoS pathogens·2025
Same author

Nanoenabling MbtI Inhibitors for Next-Generation Tuberculosis Therapy.

Journal of medicinal chemistry·2025
Same author

Author Correction: Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity.

Nature communications·2024
Same author

Catalytic peptide-based coacervates for enhanced function through structural organization and substrate specificity.

Nature communications·2024
Same journal

Catalytic synthesis of saturated azacycles using transborylation.

Organic & biomolecular chemistry·2026
Same journal

Pyridines with adamantane fragments and their 1,2,4-triazine analogues as anti-quorum-sensing agents, synthesis and molecular docking.

Organic & biomolecular chemistry·2026
Same journal

Synthesis of polymethylene-linked bis(cyclobutane-fused chromanones) mediated by gold photocatalysis.

Organic & biomolecular chemistry·2026
Same journal

Palladium-catalyzed chelation-assisted C-H functionalization of quinoline aldehydes to esters with mechanistic insights.

Organic & biomolecular chemistry·2026
Same journal

One-pot metal-free access to uracil-benzofuran bis-heterocycles: synthesis and DFT insights.

Organic & biomolecular chemistry·2026
Same journal

Transition-metal-free three-component synthesis of α-tertiary trifluoromethyl phosphonates from CF<sub>3</sub> diazo compounds.

Organic & biomolecular chemistry·2026
See all related articles

Related Experiment Video

Updated: Nov 21, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.3K

Artificial enzymes bringing together computational design and directed evolution.

Beatriz de Pina Mariz1, Sara Carvalho1, Iris L Batalha2

  • 1UCIBIO, Chemistry Department, School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal. ana.pina@fct.unl.pt.

Organic & Biomolecular Chemistry
|January 14, 2021
PubMed
Summary
This summary is machine-generated.

Artificial enzymes, or de novo enzymes, are synthetic catalysts designed for novel functions. Combining computational design with directed evolution accelerates their development for diverse industrial applications.

More Related Videos

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

237
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.2K

Related Experiment Videos

Last Updated: Nov 21, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.3K
Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

237
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.2K

Area of Science:

  • Biochemistry and Biotechnology
  • Synthetic Biology

Background:

  • Enzymes are natural protein catalysts crucial for numerous biological and industrial processes.
  • The global enzymes market is projected to reach $10.5 billion by 2024.
  • Artificial enzymes (de novo enzymes) are engineered to possess novel abiological catalytic functions.

Purpose of the Study:

  • To discuss the advantages of integrating computational design with directed evolution for creating artificial enzymes.
  • To highlight how this combined strategy overcomes individual method limitations by elucidating sequence-function relationships.
  • To review successful examples and strategies for developing artificial enzymes with significant catalytic activity.

Main Methods:

  • Computational enzyme design.
  • Directed evolution techniques.
  • Integration of computational and directed evolution approaches.

Main Results:

  • The synergistic approach provides critical insights into enzyme sequence-function relationships.
  • Demonstrated success in creating artificial enzymes with enhanced catalytic properties.
  • Enabled expansion of catalytic capabilities beyond natural enzyme limitations.

Conclusions:

  • The combination of computational design and directed evolution is a powerful strategy for de novo enzyme development.
  • This approach accelerates innovation in pharmaceuticals, chemicals, biofuels, and food industries.
  • Contributes to more sustainable development through advanced biocatalysis.