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Bifenazate induces developmental and immunotoxicity in zebrafish
Yuyang Peng1, Min Li2, Yong Huang3
1Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 330031, China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs, Affiliated Hospital of Jinggangshan University, Ji'an, 343009, China.
Chemosphere
|January 14, 2021
Summary
Bifenazate exposure harms zebrafish larvae, reducing immune cells and increasing oxidative stress. Astaxanthin supplementation can mitigate these immunotoxic and developmental effects.
Area of Science:
- Environmental Toxicology
- Immunology
- Developmental Biology
Background:
- Bifenazate is a common acaricide with unknown biological safety.
- Understanding its impact on aquatic organisms is crucial for environmental risk assessment.
Purpose of the Study:
- To investigate the immunotoxic effects of bifenazate exposure on zebrafish larvae.
- To assess the role of oxidative stress and inflammatory responses.
- To evaluate the potential protective effects of astaxanthin.
Main Methods:
- Zebrafish larvae were exposed to bifenazate.
- Developmental parameters (body length, yolk sac) were measured.
- Immune cell counts were analyzed.
- Oxidative stress markers and antioxidant gene expression were quantified.
- Inflammatory factor expression was assessed.
- Astaxanthin was used for rescue experiments.
Main Results:
- Bifenazate exposure led to reduced body length and yolk sac edema.
- Significant decrease in both innate and adaptive immune cells observed.
- Elevated oxidative stress and inhibited antioxidant activity, with altered gene expression (gclm, prdx1, serpine1, gss).
- Abnormal expression of inflammatory factors (CXCL-c1c, IFN-γ, iL-8, iL-6, MYD88).
- Astaxanthin effectively rescued bifenazate-induced developmental toxicity.
Conclusions:
- Bifenazate exhibits significant immunotoxicity in zebrafish larvae.
- Exposure induces oxidative stress and disrupts immune system function.
- Astaxanthin demonstrates potential as a countermeasure against bifenazate's adverse effects.
