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Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

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Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.9K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Related Experiment Video

Updated: Nov 21, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Model-assisted approaches for continuous chromatography: Current situation and challenges.

Dong-Qiang Lin1, Qi-Lei Zhang1, Shan-Jing Yao1

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou310027, China.

Journal of Chromatography. A
|January 14, 2021
PubMed
Summary
This summary is machine-generated.

Model-assisted approaches enhance continuous chromatography for biopharmaceutical manufacturing. These methods improve process development, control, and efficiency, supporting the shift towards continuous manufacturing.

Keywords:
Continuous chromatographyContinuous manufacturingModel-based designModel-predictive controlMonoclonal antibodyProcess modeling

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Chemical Engineering
  • Process Chemistry

Background:

  • Continuous bioprocessing offers advantages like high productivity and reduced waste.
  • Multi-column continuous chromatography is key to efficient biopharmaceutical production.
  • Traditional methods for continuous processing are time-consuming and inefficient.

Purpose of the Study:

  • To review model-assisted approaches for continuous chromatography.
  • To discuss their application in process development, validation, and control.
  • To highlight the integration of advanced modeling techniques.

Main Methods:

  • Focus on chromatographic models for multi-column systems.
  • Utilize model-assisted tools for parameter evaluation and optimization.
  • Incorporate residence time distribution models and model-predictive control.
  • Integrate artificial neural networks and machine learning for data analysis.

Main Results:

  • Model-assisted tools effectively evaluate operating parameters and identify optimal points.
  • Advanced models help understand disturbance propagation and enhance real-time monitoring.
  • Model-predictive control enables adaptive strategies for transient disturbances.
  • Machine learning improves data treatment in continuous chromatography modeling.

Conclusions:

  • Model-assisted approaches are crucial for continuous chromatography development and control.
  • Further research is urgently needed to support continuous manufacturing.
  • These methods offer significant improvements over traditional experimental approaches.