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Developing Hybrid Polymer Scaffolds Using Peptide Modified Biopolymers for Cell Implantation.

Sinoj Abraham1, Purushothaman Kuppan, Shammy Raj1

  • 1IngenuityLab, National Institute for Nanotechnology, 11421 Saskatchewan Drive NW, Edmonton, Alberta T6G 2M9, Canada.

ACS Biomaterials Science & Engineering
|January 15, 2021
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Summary

This study developed a novel hybrid scaffold using biodegradable polymers to prevent foreign body reactions and maintain cell viability. This biomaterial approach significantly improved neo-vascularization and islet cell survival in transplantation studies.

Keywords:
biomimicsbiopolymersubcutaneoustransplantationvascularization

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cellular Therapy

Background:

  • Polymeric scaffolds with biomimics show promise for cellular therapies.
  • Existing methods face challenges with foreign body reactions and cell viability.
  • Improving scaffold integration is crucial for successful cell transplantation.

Purpose of the Study:

  • To design and fabricate a hybrid scaffold that prevents foreign body reactions.
  • To maintain cell viability within the scaffold for enhanced therapeutic outcomes.
  • To investigate the potential of this scaffold in improving cell transplantation success rates.

Main Methods:

  • Developed a biodegradable, acrylic-based, cross-linkable polycaprolactone polymer.
  • Fabricated hybrid scaffolds using a multihead electrospinning station.
  • Incorporated cell growth factor mimics and anti-foreign body reaction factors into the scaffold.

Main Results:

  • Transplantation studies in immuno-competent and immuno-suppressed mice demonstrated extensive neo-vascularization.
  • Maintained neonatal porcine islet cell viability for up to 20 weeks post-transplant in subcutaneous implants.
  • The scaffold successfully prevented adverse foreign body reactions.

Conclusions:

  • This novel hybrid scaffold approach enhances revascularization in cell transplantation.
  • The scaffold design supports long-term cell viability and integration.
  • This technology offers a promising strategy for improving cellular therapies.