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Self-reporting Scaffolds for 3-Dimensional Cell Culture

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Cell Microenvironment pH Sensing in 3D Microgels Using Fluorescent Carbon Dots.

Anil Chandra¹, Neetu Singh^1,2

¹Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi-110016, India.

ACS Biomaterials Science & Engineering

|January 15, 2021

Summary

This summary is machine-generated.

This study introduces a 3D cell culture system using microgels with pH-sensitive carbon dots to monitor cellular microenvironment changes. This biocompatible platform enables real-time tracking of cell growth and disease progression via fluorescence.

Keywords:

3D cell culture carbon dots microenvironment microgel pH sensing

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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Area of Science:

Biomaterials Science
Cell Biology
Microfluidics

Background:

The cellular microenvironment's pH is crucial for biological processes.
Monitoring pH changes in 3D cell cultures is challenging.
Existing methods lack spatial control and real-time monitoring capabilities.

Purpose of the Study:

To develop a 3D cell culture microgel system for sensing cellular microenvironment pH changes.
To create a biocompatible scaffold for cell proliferation and pH monitoring.
To utilize fluorescent carbon dots for real-time pH tracking.

Main Methods:

Utilized droplet-based microfluidics to encapsulate cells and pH-sensitive carbon dots in polyethylene glycol microgels.
Synthesized green-fluorescent carbon dots with pH sensitivity in the physiological range (5.8-7.7).
Employed fluorescence microscopy to monitor pH-induced fluorescence changes in the microgels.

Main Results:

Demonstrated a biocompatible 3D microgel system supporting mammalian cell proliferation.
Showcased pH-sensitive carbon dots that exhibit increased fluorescence with decreasing pH.
Successfully monitored pH changes in the cellular microenvironment correlated with cell growth.

Conclusions:

The developed microgel system with carbon dots offers a promising platform for studying dynamic changes in the cellular microenvironment.
This technology can be applied to monitor cell growth, disease progression, and other pH-dependent biological processes in real-time.
The microfluidics approach allows for customizable microgel size, composition, and architecture for complex scaffold development.