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A Salt Stimulus-Responsive Nanohydrogel for Controlled Fishing Low-Density Lipoprotein with Superior Adsorption
Jian-Fang Cao1, Wang Xu1, Yao-Yao Zhang1
1Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.
ACS Applied Materials & Interfaces
|January 15, 2021
Summary
A novel salt-responsive nanoplatform using zwitterionic nanohydrogels and chondroitin sulfate effectively captures low-density lipoprotein (LDL). This platform demonstrates high adsorption capacity and selectivity, enabling efficient LDL separation from serum for hypercholesterolemia applications.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Hypercholesterolemia poses significant health risks, necessitating efficient methods for low-density lipoprotein (LDL) detection and removal.
- Current separation techniques often lack selectivity and efficiency, driving the need for advanced nanoplatforms.
Purpose of the Study:
- To develop a salt-responsive nanoplatform for the selective capture and separation of LDL.
- To investigate the salt-induced swelling and chondroitin sulfate (CS) exposure mechanism for enhanced LDL adsorption.
Main Methods:
- Construction of a carboxylated silica (SiO2-COOH) framework functionalized with zwitterionic nanohydrogels (NGs).
- Modification of NGs with chondroitin sulfate (CS) via amidation for specific LDL recognition.
- Evaluation of salt-responsive swelling, LDL adsorption capacity, selectivity, and stripping efficiency using MgCl2 and SDS.
Main Results:
- The SiO2-NGs-CS nanoplatform exhibited enhanced swelling and CS exposure in saline environments due to an anti-polyelectrolyte effect.
- Achieved controlled LDL adsorption (7.2-93%) by adjusting MgCl2 concentration, with a maximum adsorption capacity of 898.1 mg g-1.
- Demonstrated superior selectivity for LDL over other proteins and efficient LDL recovery (95.4%) using SDS.
Conclusions:
- The developed salt-responsive nanoplatform offers a promising strategy for highly efficient and selective LDL separation.
- This technology has potential applications in diagnosing and managing hypercholesterolemia by isolating LDL from biological samples.
