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Updated: Nov 21, 2025

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Gold Nanoparticle-Redox Ionic Liquid based Nanoconjugated Matrix as a Novel Multifunctional Biosensing Interface.

Kandaswamy Theyagarajan1, Sangeeta Yadav2,3, Jitendra Satija3

  • 1Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore 632014, India.

ACS Biomaterials Science & Engineering
|January 15, 2021
PubMed
Summary

A novel nanoconjugated biosensor was developed for sensitive electrochemical detection of hydrogen peroxide (H2O2). This advanced interface utilizes a functionalized ionic liquid and gold nanoparticles, offering improved stability and performance for real-time monitoring.

Keywords:
electrochemical biosensorgold nanoparticleshorseradish peroxidasehydrogen peroxidenanobioconjugateredox ionic liquid

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Area of Science:

  • Electrochemistry
  • Biosensor Development
  • Nanomaterials

Background:

  • Effective immobilization of biomolecules is crucial for constructing robust biosensors.
  • Real-time monitoring requires sensitive and stable electrochemical interfaces.
  • Hydrogen peroxide (H2O2) detection is vital in various biological and environmental applications.

Purpose of the Study:

  • To develop a novel nanoconjugated biosensing interface for electrochemical H2O2 determination.
  • To create a stable and sensitive platform by immobilizing horseradish peroxidase (HRP) onto a functionalized ionic liquid and gold nanoparticle matrix.
  • To investigate the electrochemical properties and sensing capabilities of the developed HRP-CHO-AIL/AuNPs/GCE.

Main Methods:

  • Synthesis of an anthracene-substituted, aldehyde-functionalized redox ionic liquid (CHO-AIL).
  • Covalent immobilization of horseradish peroxidase (HRP) onto CHO-AIL on a glassy carbon electrode (GCE).
  • Electrostatic tethering of anionic gold nanoparticles (AuNPs) to the HRP-CHO-AIL matrix.
  • Electrochemical characterization and H2O2 detection using cyclic voltammetry and amperometry.

Main Results:

  • The HRP-CHO-AIL/GCE exhibited stable redox peaks for the anthracene moiety.
  • The HRP-CHO-AIL/AuNPs/GCE showed enhanced electron transfer, indicated by a shift in formal potential and increased peak currents.
  • The developed biosensor demonstrated a linear response to H2O2 in the range of 0.02-2.77 mM with a low detection limit of 3.7 μM.
  • The biosensor exhibited excellent selectivity, sensitivity, stability, and reproducibility.

Conclusions:

  • The designed nanobioconjugate, HRP-CHO-AIL/AuNPs/GCE, provides an effective platform for sensitive and stable electrochemical detection of H2O2.
  • The synergistic integration of the functionalized ionic liquid and gold nanoparticles significantly enhances biosensor performance.
  • This nanoconjugated interface is suitable for practical applications requiring real-time H2O2 monitoring.