Intraocular Injection of HyStem Hydrogel Is Tolerated Well in the Rabbit Eye

Area of Science:

• Ophthalmology
• Biomaterials Science
• Regenerative Medicine

Background:

• HyStem hydrogel is a potential carrier for ocular cell or drug delivery.
• Its solidification properties necessitate evaluation for intraocular use.
• Understanding its long-term biocompatibility is crucial for ophthalmic applications.

Purpose of the Study:

• To assess the long-term biocompatibility and safety of HyStem hydrogel in the rabbit eye.
• To evaluate HyStem hydrogel as a potential carrier for ocular delivery.
• To determine the ocular disposition and clearance of HyStem hydrogel.

Main Methods:

• Fluorescein-labeled HyStem hydrogel was injected into the vitreous space of rabbit eyes.
• Ocular disposition was monitored using fluorescence fundus photography.
• Retinal morphology was assessed via optical coherence tomography (OCT) and fundus photography.
• Visual function was evaluated using electroretinography (ERG).
• Immunohistochemistry was performed to detect inflammatory markers.

Main Results:

• HyStem hydrogel was successfully delivered without forming retinal deposits.
• No retinal inflammation was observed through OCT, fundus imaging, and immunohistochemistry.
• Electroretinography showed no adverse impact on retinal function.
• The hydrogel's clearance half-time from the posterior chamber was 3.9 days.

Conclusions:

• HyStem hydrogel is biocompatible for long-term intraocular application in large animal eyes.
• The hydrogel is safe for use as a carrier in ocular drug or cell delivery.
• Further research can explore HyStem hydrogel's therapeutic potential in ophthalmology.