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Updated: Nov 21, 2025

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

GABA quantification in human anterior cingulate cortex.

Jan Weis¹, Jonas Persson², Andreas Frick³

¹Department of Medical Physics, Uppsala University Hospital, Uppsala, Sweden.

|January 15, 2021

Summary

This summary is machine-generated.

This study introduces a new method for quantifying brain gamma-aminobutyric acid (GABA) using PRESS and MEGA-PRESS MRI. The findings suggest this approach enhances GABA estimation reliability with metabolite references.

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Published on: April 15, 2015

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Published on: July 17, 2011

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Last Updated: Nov 21, 2025

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

Investigating the Function of Deep Cortical and Subcortical Structures Using Stereotactic Electroencephalography: Lessons from the Anterior Cingulate Cortex

Investigating the Function of Deep Cortical and Subcortical Structures Using Stereotactic Electroencephalography: Lessons from the Anterior Cingulate Cortex

Published on: April 15, 2015

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Published on: July 17, 2011

Area of Science:

Neuroscience
Magnetic Resonance Imaging
Biochemistry

Background:

Gamma-aminobutyric acid (GABA) is a key inhibitory neurotransmitter in the human brain.
Altered GABA levels are implicated in various neurological and psychiatric disorders, necessitating accurate quantification methods.

Purpose of the Study:

To propose and validate a combined PRESS and MEGA-PRESS magnetic resonance imaging (MRI) acquisition strategy for absolute GABA quantification.
To compare GABA concentration estimates derived from metabolite references (tCho, tCr, tNAA) against water-referenced quantification.
To demonstrate the efficacy of the QuasarX algorithm with a specific in vitro basis set for fitting MEGA-PRESS spectra.

Main Methods:

Utilized a 3T MRI scanner with MEGA-PRESS sequences for GABA quantification in 13 volunteers.
Employed interleaved water referencing for real-time B0 drift correction and pulse frequency adjustments.
Acquired reference metabolite concentrations using PRESS sequences and corrected absolute concentrations for tissue water fractions and relaxation effects.

Main Results:

Water-referenced GABA estimations were significantly higher than those obtained using metabolite references (tCho, tCr, tNAA).
The QuasarX algorithm, combined with an in vitro basis set, improved the reliability of GABA+ spectral fitting.
The proposed method allows for the use of tCho, tCr, and tNAA as internal concentration references for GABA quantification.

Conclusions:

The developed GABA quantification method using PRESS and MEGA-PRESS acquisitions reliably utilizes metabolite references.
This approach holds significant potential for improving the accuracy and reliability of GABA estimation in clinical and research settings.
The QuasarX algorithm enhances spectral fitting, contributing to more dependable GABA quantification.