Towards improving ELISA surfaces: SPR assessment of polystyrene modification efficiency for promoting immobilization
Gennadii Beketov1, Olena Shynkarenko1, Mariia Apatska1
1V.E. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine., Nauki Ave. 41, 03028, Kiev, Ukraine.
Abstract:
The enzyme-linked immunosorbent assay (ELISA) is a widely used diagnostic technique. In ELISA, detection of the target biomolecules is achieved through selective capture by appropriate antibody immobilized on a solid support. Our study addresses the application of surface plasmon resonance to an assessment of the polystyrene modification efficiency for promoting adsorption of biomolecules. A method facilitating the development of advanced immobilization strategies for biofunctionalization of polystyrene surface was evolved. The proposed approach uses formation of a thin layer of polystyrene over the SPR chip surface, thus enabling a detailed characterization of biomolecular interactions at the polystyrene surface.
