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Updated: Nov 21, 2025

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Synthesis and chromatographic evaluation of pyrazinedicarboxylic anhydride bonded stationary phase
Gaowa Jin1, Junjie Ding2, Yongzheng Zhou3
1CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Jiangxi Chinese medicine science center of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Nanchang 330100, China.
Abstract:
A silica based hydrophilic stationary phase bonding with 2,3-pyrazinedicarboxylic anhydride and amino groups was synthesized via amino-acid anhydride ring opening reaction. The bonded groups could not only provide hydrophilic interaction, but also electrostatic, π-π and hydrogen bonding interactions, etc. The results of characterization with elemental analysis and solid-state 13C cross-polarization magic-angle-spinning NMR indicated the successful preparation of amino and carboxyl bonded stationary phase named ZAC. The ζ-potential of ZAC stationary phase showed the negatively charge was dominate at pH larger than 3.5. Chromatographic evaluation revealed that ZAC stationary phase behaved well under HILIC mode. It showed different selectivity and retention compared to some typical commercial columns, and it was validated by the separation of chitooligosaccharides, flavonoid glycosides, organic acids and alkaloid samples. Based on the different selectivity between ZAC stationary phase and C18 columns, ZAC stationary phase also showed different selectivity with C18. And it was verified by the separation of Lonicerae Japonicae Flos and Menispermi Rhizoma extracts.
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