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Extending In-Plane Impedance Measurements from 2D to 3D Cultures: Design Considerations.

Sorel E De Leon1,2, Lana Cleuren3, Zay Yar Oo1,2

  • 1Bioengineering Research Group, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.

Bioengineering (Basel, Switzerland)
|January 16, 2021
PubMed
Summary
This summary is machine-generated.

Impedance spectroscopy monitors cells in 3D cultures. While effective for 2D models, current electrode designs struggle to detect cell changes in 3D collagen gels, highlighting the need for improved characterization techniques.

Keywords:
3D cell culturecell proliferationelectrical impedance spectroscopyin situ monitoringtissue culture

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioimpedance

Background:

  • Three-dimensional (3D) cell cultures offer advanced biological models.
  • Real-time, label-free cell monitoring techniques are needed for 3D cultures.
  • Impedance spectroscopy is a promising label-free technique for cell monitoring.

Purpose of the Study:

  • Investigate the full impedance spectrum of human fibroblast cells in 2D and 3D collagen gel cultures.
  • Determine how system components affect impedance signals in 3D cell models.
  • Evaluate impedance spectroscopy for real-time monitoring of cells in 3D cultures.

Main Methods:

  • Utilized impedance spectroscopy across a wide frequency range (10 Hz to 5 MHz).
  • Employed a commercial well with in-plane electrodes for human fibroblast cell cultures.
  • Compared impedance measurements in 2D cultures versus 3D collagen gel cultures.

Main Results:

  • Protein adsorption impacted impedance in cell-free samples at low frequencies, an effect mitigated by cell presence.
  • Cell proliferation was detectable in 2D cultures at intermediate frequencies (30 kHz).
  • In-plane electrodes failed to detect impedance changes in 3D collagen gel cultures with cells.

Conclusions:

  • Electrode architecture and cell distribution are critical for impedance-based monitoring in 3D cultures.
  • Current in-plane electrode designs are insufficient for detecting cellular changes in 3D collagen gels.
  • Further development of impedance measurement devices is required for effective 3D cell culture characterization.