Multifunctional Chitosan/Polycaprolactone Nanofiber Scaffolds with Varied Dual-Drug Release for Wound-Healing...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Nanotechnology

Background:

• Advanced wound dressings are crucial for trauma care, requiring hemostasis, antibacterial action, and therapeutic effects.
• Electrospinning offers a versatile platform for creating multifunctional wound dressings.

Purpose of the Study:

• To design and evaluate lidocaine hydrochloride (LID) and mupirocin-loaded chitosan/polycaprolactone (CSLD-PCLM) nanofibrous scaffolds as advanced wound dressings.
• To assess the hemostatic, antibacterial, drug release, and wound healing capabilities of the CSLD-PCLM scaffolds.

Main Methods:

• Dual spinneret electrospinning was employed to fabricate CSLD-PCLM nanofibrous scaffolds.
• In vitro drug release kinetics for LID and mupirocin were analyzed.
• In vitro antibacterial activity against relevant pathogens was evaluated.
• In vivo wound healing was assessed in a full-thickness skin defect model.

Main Results:

• The CSLD-PCLM scaffolds exhibited a nanofiber structure promoting blood cell interaction and excellent blood coagulation.
• Rapid release of LID and sustained release of mupirocin were observed.
• The mupirocin-containing scaffolds demonstrated significant antibacterial activity.
• In vivo studies showed enhanced wound healing with complete re-epithelialization and collagen deposition.

Conclusions:

• CSLD-PCLM nanofibrous scaffolds possess multifunctional properties suitable for advanced wound dressings.
• These scaffolds effectively promote hemostasis, exhibit antibacterial action, and facilitate wound healing.
• CSLD-PCLM scaffolds represent promising candidates for clinical applications in trauma and wound management.