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Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

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Acoustofluidic phase microscopy in a tilted segmentation-free configuration.

Julián Mejía Morales, Björn Hammarström, Gian Luca Lippi¹

¹Institut de Physique de Nice, Université Côte d'Azur, CNRS, 06560 Valbonne, France.

Biomicrofluidics

|January 18, 2021

Summary

This summary is machine-generated.

This study introduces a low-cost quantitative phase microscopy (QPM) device that uses acoustic focusing for cell alignment and imaging in continuous flow, enabling registration-free phase map generation.

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Area of Science:

Biomedical Optics
Microscopy
Cellular Imaging

Background:

Quantitative Phase Microscopy (QPM) is valuable for label-free cell analysis.
Traditional QPM methods often require complex object segmentation and registration.
Continuous flow systems offer advantages for high-throughput biological sample analysis.

Purpose of the Study:

To develop a low-cost, registration-free QPM device for cells in continuous flow.
To enable label-free phase map generation of individual cells without object segmentation.
To demonstrate the device's utility in biological imaging applications.

Main Methods:

Utilized acoustic focusing to align cells in a single plane with constant velocity.
Employed an inclined geometry between the acoustic focusing plane and microscope focal plane.
Acquired multi-focal plane images without mechanical scanning for phase map reconstruction.

Main Results:

Successfully generated phase maps of cells without requiring segmentation or registration.
Demonstrated a cost-effective and robust implementation of QPM.
Validated the system on blood samples, recovering phase maps of individual red blood cells.

Conclusions:

The developed low-cost QPM device effectively images cells in continuous flow.
The registration-free approach simplifies QPM data processing.
This method shows promise for efficient and affordable biological sample analysis.