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Updated: Nov 21, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
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Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

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SMRT-based mitochondrial genome of the edible mushroom Morchella conica

Wei Li1, Fen Zhang1, Li-Zhi Gao1,2

  • 1Institution of Genomics and Bioinformatics, South China Agricultural University, Guangzhou, China.

Mitochondrial DNA. Part B, Resources
|January 18, 2021
PubMed

Insights

The complete mitochondrial genome of the prized edible mushroom, Morchella conica, was sequenced. This research provides a foundation for genetic breeding and understanding the evolution of this valuable fungus.

Area of Science:

  • Mycology
  • Genomics
  • Fungal Biology

Background:

  • Morchella conica is a highly valued mushroom with significant edible and medicinal properties.
  • Understanding its genetic makeup is crucial for its cultivation and utilization.

Purpose of the Study:

  • To determine the complete mitochondrial genome sequence of Morchella conica.
  • To analyze its genomic features and perform phylogenetic analysis.

Main Methods:

  • Combined PacBio and Illumina sequencing technologies for genome determination.
  • Bioinformatic analysis to identify genes and non-conserved open reading frames (ncORFs).
  • Phylogenetic analysis with other fungal species from Ascomycota, Basidiomycota, and Mucoromycota.

Main Results:

  • The complete mitochondrial genome of M. conica is 280,763 bp with a GC content of 39.88%.
  • Identified 14 core conserved protein-coding genes, 127 ncORFs, and 30 tRNA genes.
  • No large or small ribosomal RNA subunits (rnl or rns) were found; two mitochondrial RNase P (rnpB) and one ribosomal protein (rps3) genes were detected.
  • Phylogenetic analysis indicated M. conica is most closely related to M. importuna.

Conclusions:

  • The sequenced M. conica mitochondrial genome provides essential data for genetic breeding programs.
  • This genomic information enhances the understanding of M. conica's evolutionary relationships within fungi.
Keywords:
Morchella conicaPacBioevolutionmitochondrial genomemushroom

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