Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of Amides01:29

Preparation of Amides

3.7K
Amides are synthesized by treating carboxylic acids with amines in the presence of dehydrating agents like dicyclohexylcarbodiimide (DCC).
The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
Subsequently, the amine acts as a nucleophile that attacks the acylating agent to form a tetrahedral intermediate. In the...
3.7K
Adrenergic Agonists: Chemistry and Structure-Activity Relationship01:16

Adrenergic Agonists: Chemistry and Structure-Activity Relationship

3.6K
Adrenergic agonists' structure-activity relationship (SAR) determines their selectivity and efficacy. These agonists comprise a phenylethylamine moiety with an aromatic ring and an ethylamine side chain.
Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
Separation of...
3.6K
Amines: Introduction01:07

Amines: Introduction

5.1K
Amines are organic derivatives of ammonia. They are formed by replacing one or more ammonia protons with alkyl or aryl groups. Depending upon the number of organyl groups bonded to nitrogen, amines are classified as primary, secondary, or tertiary. Primary amines have one organyl group attached to the nitrogen atom, while secondary and tertiary amines have two and three organyl groups attached to the nitrogen atom, respectively.
5.1K
Physical Properties of Amines01:26

Physical Properties of Amines

3.8K
Amines with low molecular weight are usually gaseous at room temperature, while those with high molecular weight are liquid or solids in nature. Usually, low molecular weight amines have a rotten fish-like smell. Diamines typically have a pungent smell. For instance, cadaverine and putrescine, depicted in Figure 1, are two molecules responsible for decaying tissue.
3.8K
Nomenclature of Carboxylic Acid Derivatives: Amides and Nitriles01:11

Nomenclature of Carboxylic Acid Derivatives: Amides and Nitriles

4.8K
Naming Amides
The IUPAC and common names of amides are derived from the parent carboxylic acid, by replacing the suffix “oic acid” and “ic acid,” respectively, with “amide.” In the following example, the IUPAC name ethanamide is derived from ethanoic acid, and the common name, acetamide, is obtained from acetic acid.
4.8K
Amines to Amides: Acylation of Amines01:19

Amines to Amides: Acylation of Amines

3.0K
Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three new compounds from <i>Nardostachys jatamansi</i>.

Journal of Asian natural products research·2026
Same author

Three new compounds including a phenolic acid, a flavonoid, and a benzophenone from the whole plants of <i>Hedyotis diffusa</i>.

Journal of Asian natural products research·2026
Same author

Empirical rules for efficiently determining the structures and configurations of natural compounds via 1D NMR.

Journal of Asian natural products research·2026
Same author

Identification and field evaluation of sex pheromone components in the pine tip moth, Dioryctria sylvestrella (Lepidoptera: Pyralidae).

Pest management science·2026
Same author

Antidiabetic Polyphenolic Compounds Including Two Unique Dimers Featuring a 6/6/7/6/5 Ring System from the Roots of <i>Sophora tonkinensis</i>.

Journal of natural products·2026
Same author

Effects of minor pheromone components on orientation behavior and mating decision-making strategy in Micromelalopha sieversi.

Insect science·2026

Related Experiment Video

Updated: Nov 21, 2025

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
07:36

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Published on: December 23, 2022

1.7K

Bioactive amides from Polygonum cuspidatum.

Xu Zhang1, Fu Liu2, Zi-Ming Feng1

  • 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Journal of Asian Natural Products Research
|January 18, 2021
PubMed
Summary
This summary is machine-generated.

Two new amide enantiomers and two known amides were isolated from Polygonum cuspidatum. Compounds 2 and 3 demonstrated significant alpha-glucosidase inhibitory activity, suggesting potential therapeutic applications.

Keywords:
PolygonaceaePolygonum cuspidatumamidesα-glucosidase inhibition

More Related Videos

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.7K
Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
07:50

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

873

Related Experiment Videos

Last Updated: Nov 21, 2025

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
07:36

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Published on: December 23, 2022

1.7K
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.7K
Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
07:50

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

873

Area of Science:

  • Natural Product Chemistry
  • Pharmacology
  • Medicinal Chemistry

Background:

  • Polygonum cuspidatum (Japanese knotweed) is a traditional medicinal herb.
  • Its rhizomes are a rich source of bioactive compounds.
  • Investigating these compounds may yield novel therapeutic agents.

Purpose of the Study:

  • To isolate and characterize new and known amide compounds from Polygonum cuspidatum rhizomes.
  • To elucidate the chemical structures and absolute configurations of the isolated compounds.
  • To evaluate the alpha-glucosidase inhibitory potential of these compounds.

Main Methods:

  • Phytochemical investigation of Polygonum cuspidatum rhizomes.
  • Structure elucidation using UV, IR, High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS), and Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Determination of absolute configurations via Electronic Circular Dichroism (ECD) method.
  • In vitro assay for alpha-glucosidase inhibitory activity.

Main Results:

  • One pair of new amide enantiomers (1a and 1b) and two known amides were successfully isolated.
  • Compound 1 features a unique C-C linkage between feruloyltyramine and resveratrol moieties.
  • Compounds 2 and 3 exhibited significant alpha-glucosidase inhibition with IC50 values of 2.82 and 13.06 μmol/L, respectively, outperforming the positive control acarbose (IC50 385 μmol/L).

Conclusions:

  • The study successfully identified novel amide compounds from Polygonum cuspidatum.
  • The isolated compounds, particularly 2 and 3, show promising alpha-glucosidase inhibitory effects.
  • These findings highlight the potential of Polygonum cuspidatum-derived compounds for managing conditions related to alpha-glucosidase activity, such as type 2 diabetes.