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Genetic engineering in organoids
Isaree Teriyapirom1, Andreia S Batista-Rocha2, Bon-Kyoung Koo3
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria. isaree.teriyapirom@imba.oeaw.ac.at.
Journal of Molecular Medicine (Berlin, Germany)
|January 18, 2021
Summary
Three-dimensional organoids derived from stem cells are valuable for modeling development and disease. This review summarizes genetic manipulation methods for organoids, highlighting their pros and cons to guide optimal strategy design.
Area of Science:
- Stem cell biology
- Developmental biology
- Genetics
Background:
- Three-dimensional organoids are widely used for developmental and disease modeling.
- Organoids are derived from adult and pluripotent stem cells, mimicking human and mouse organs.
- Established culture protocols exist for differentiation and expansion.
Purpose of the Study:
- To review and summarize various genetic manipulation methods applicable to organoids.
- To provide a comparative analysis of the pros and cons of each method.
- To assist researchers in designing optimal genetic engineering strategies for organoids.
Main Methods:
- Literature review of existing organoid genetic manipulation techniques.
- Comparative analysis of different genetic engineering approaches.
- Discussion of standardization needs for organoid genetics.
Main Results:
- Identification of diverse genetic manipulation methods for organoids.
- Evaluation of the advantages and disadvantages of each technique.
- Highlighting areas where standardization is needed.
Conclusions:
- Genetic manipulation methods for organoids are diverse but require standardization.
- Understanding the pros and cons of each method is crucial for effective organoid engineering.
- This review provides a framework for selecting optimal genetic strategies in organoid research.
