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Related Experiment Video

Updated: Nov 21, 2025

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
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Lung organoids: advances in generation and 3D-visualization.

Brian Cunniff1, Joseph E Druso1, Jos L van der Velden2

  • 1Department of Pathology and Laboratory Medicine, University of Vermont Cancer Center, Larner College of Medicine, HSRF Bldg., Rm. 214A, Burlington, VT, 05405, USA.

Histochemistry and Cell Biology
|January 18, 2021
PubMed
Summary

Lung organoids offer a 3D model to study lung diseases. These advanced models maintain tissue architecture and cellular functions lost in traditional 2D cultures, improving lung research.

Keywords:
Advanced microscopyLung biologyLung organoidsOrganoid methodologies

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Area of Science:

  • Pulmonary biology and regenerative medicine.

Background:

  • The lung's complex 3D structure, composed of over 40 cell types, is crucial for efficient function.
  • Disruption of lung architecture is linked to disease development.
  • Traditional 2D cell cultures fail to replicate in vivo cellular and molecular responses, losing critical gene-expression patterns.

Purpose of the Study:

  • To highlight the need for advanced technologies to study lung function and disease in a 3D context.
  • To introduce lung organoids as a novel model system for investigating lung biology ex vivo.
  • To discuss emerging technologies for lung organoid culture, visualization, and application in disease research.

Main Methods:

  • Culturing airway cell-derived organoids that mimic lung structure and function.
  • Utilizing high-resolution microscopy for detailed visualization of organoid structures.
  • Analyzing data derived from organoids to understand lung physiology and pathology.

Main Results:

  • Lung organoids successfully recapitulate key aspects of lung architecture and function ex vivo.
  • Organoids provide a platform to study cellular and molecular responses under conditions that preserve 3D tissue complexity.
  • Emerging technologies enable detailed investigation of lung biology using organoid models.

Conclusions:

  • Lung organoids represent a significant advancement in modeling lung biology and disease.
  • These 3D models overcome limitations of 2D cultures, facilitating a more complete understanding of lung function.
  • Organoid technology is poised to drive progress in lung disease research and therapeutic development.