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STED lithography in microfluidics for 3D thrombocyte aggregation testing.

Bianca Buchegger1,2, Alexander Tanzer1, Sandra Posch3

  • 1Institute of Applied Physics and Linz Institute of Technology (LIT), Johannes Kepler University Linz, Altenberger Straße 69, 4040, Linz, Austria.

Journal of Nanobiotechnology
|January 19, 2021
PubMed
Summary
This summary is machine-generated.

This study integrates microfluidics with 3D photopolymerization, creating nanoanchors to control von Willebrand factor (vWF) density. Thrombocyte activation decreased as vWF density lowered on these 3D scaffolds.

Keywords:
MicrofluidicsMultiphoton polymerization lithographyStimulated emission depletion lithographyThrombocyte activationVon Willebrand factor

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cell Biology

Background:

  • Advanced 3D photopolymerization techniques like multiphoton polymerization lithography (MPL) and stimulated emission depletion (STED) lithography enable sub-micrometer structure fabrication.
  • Integrating microfluidics with these techniques expands their application scope, particularly in biological studies.

Purpose of the Study:

  • To develop a microfluidic device featuring 3D MPL structures with STED-lithographically defined nanoanchors.
  • To investigate the impact of von Willebrand factor (vWF) density on thrombocyte activation within a microfluidic environment.

Main Methods:

  • Fabrication of 3D microfluidic structures using multiphoton polymerization lithography (MPL).
  • Writing of nanoanchors onto 3D structures via stimulated emission depletion (STED) lithography.
  • Controlled immobilization of von Willebrand factor (vWF) by adjusting nanoanchor density.
  • Assessment of thrombocyte activation in response to varying vWF densities within the microfluidic device.

Main Results:

  • Successfully created a microfluidic device with 3D MPL structures and STED-written nanoanchors.
  • Demonstrated the ability to precisely control vWF density by varying nanoanchor numbers.
  • Observed a trend of decreased thrombocyte activation with reduced vWF density on the 3D scaffolds.

Conclusions:

  • The developed microfluidic platform allows for controlled investigation of cell-surface interactions.
  • Findings suggest that lower densities of vWF on 3D scaffolds may reduce thrombocyte activation.
  • This approach offers a novel method for studying thrombocyte behavior in a physiologically relevant context.