Determination of acetamiprid using electrochemiluminescent aptasensor modified by MoS₂QDs-PATP/PTCA and NH₂-UiO-66

A novel aptasensor has been fabricated based on the resonance energy transform (RET) system from MoS₂QDs-PATP/PTCA (donor) to NH₂-UiO-66 (acceptor). The electrochemiluminescence (ECL) signal of PTCA was greatly amplified due to the decoration of MoS₂QDs-PATP, and the NH₂-UiO-66 was utilized to label the signal probe DNA (pDNA), which hybridizes with the exposed aptamer anchored on the surface of MoS₂QDs-PATP/PTCA. With the target acetamiprid, the specific binding of acetamiprid to aptamer causes the connection between the donor and the acceptor to be interrupted and produce an "on" ECL signal. Thus, an "off-on" ECL sensing platform for sensitive and selective acetamiprid assay was designed. Under the optimal condition, the ECL signal of the aptasensor was found to be linearly related to the logarithm of the acetamiprid concentration ranging from 0.1 fM to 0.1 μM with a detection limit of 0.064 fM. More importantly, the recovery rate of the ECL aptasensor was calculated to be 98.7 ~ 106% with a RSD lower 5.1% for the residual acetamiprid assay in real food samples, which indicated that the aptasensor has high potential for practical applications.