Competition for electrons favours N₂ O reduction in denitrifying Bradyrhizobium isolates

Bradyrhizobia are common members of soil microbiomes and known as N₂ -fixing symbionts of economically important legumes. Many are also denitrifiers, which can act as sinks or sources for N₂ O. Inoculation with compatible rhizobia is often needed for optimal N₂ -fixation, but the choice of inoculant may have consequences for N₂ O emission. Here, we determined the phylogeny and denitrification capacity of Bradyrhizobium strains, most of them isolated from peanut-nodules. Analyses of genomes and denitrification end-points showed that all were denitrifiers, but only ~1/3 could reduce N₂ O. The N₂ O-reducing isolates had strong preference for N₂ O- over NO₃ ^- -reduction. Such preference was also observed in a study of other bradyrhizobia and tentatively ascribed to competition between the electron pathways to Nap (periplasmic NO₃ ^- reductase) and Nos (N₂ O reductase). Another possible explanation is lower abundance of Nap than Nos. Here, proteomics revealed that Nap was instead more abundant than Nos, supporting the hypothesis that the electron pathway to Nos outcompetes that to Nap. In contrast, Paracoccus denitrificans, which has membrane-bond NO₃ ^- reductase (Nar), reduced N₂ O and NO₃ ^- simultaneously. We propose that the control at the metabolic level, favouring N₂ O reduction over NO₃ ^- reduction, applies also to other denitrifiers carrying Nos and Nap but lacking Nar.