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Sardine Roe as a Source of Lipids To Produce Liposomes.

Marta Guedes1,2, Ana R Costa-Pinto3, Virgínia M F Gonçalves4

  • 13B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal.

ACS Biomaterials Science & Engineering
|January 19, 2021
PubMed
Summary

Sardine roe lipids were extracted and used to create novel multilamellar liposomes (MLVs) for drug delivery. These MLVs, particularly those from MTBE extracts, showed good cytocompatibility, indicating potential for biomedical applications.

Keywords:
Bligh and Dyercytocompatibilityfatty acidsliposomesmethyl-tert-butyl ethersardine roe

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Area of Science:

  • Biomaterials Science
  • Marine Biotechnology
  • Drug Delivery Systems

Background:

  • Sea-derived materials offer significant potential in medical, pharmaceutical, and biotechnological applications.
  • Fish roe, specifically sardine roe (Sardina pilchardus), is a nutrient-rich source with known health benefits.
  • Exploring novel sources for drug delivery systems is crucial for advancing biomedical treatments.

Purpose of the Study:

  • To investigate sardine roe lipids as a source for novel drug delivery systems.
  • To characterize sardine roe lipids and their suitability for producing multilamellar liposomes (MLVs).
  • To evaluate the drug-releasing capabilities and cytocompatibility of MLVs loaded with celecoxib.

Main Methods:

  • Sardine roe underwent morphological, histological, and histochemical analysis.
  • Lipid extraction was performed using Bligh and Dyer (BD) and methyl-tert-butyl ether (MTBE) methods.
  • Lipid extracts were analyzed using gas chromatography/mass spectrometry, and MLVs were formulated and characterized for size, surface charge, and drug release.

Main Results:

  • Sardine roe lipid extracts were rich in fatty acids, including omega-3 polyunsaturated fatty acids.
  • Multilamellar liposomes (MLVs) were successfully produced, exhibiting size heterogeneity and negative surface charge.
  • MLVs formulated with MTBE lipid extracts demonstrated superior cytocompatibility compared to those from the BD method.
  • Controlled release of the anti-inflammatory drug celecoxib was observed from the MLVs.

Conclusions:

  • Sardine roe is a viable source for obtaining lipids suitable for creating advanced drug delivery systems.
  • Lipid extracts obtained via MTBE method yield MLVs with enhanced cytocompatibility.
  • Further chemical processing of lipid extracts can potentially improve the performance of these novel drug delivery systems for biomedical applications.