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Updated: Nov 20, 2025

Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
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Metabolome-Driven Regulation of Adenovirus-Induced Cell Death

Anastasia Laevskaya1, Anton Borovjagin2, Peter S Timashev3,4,5

  • 1Group of Experimental Biotherapy and Diagnostic, Institute for Regenerative Medicine, World-Class Research Center "Digital Biodesign and Personalized Healthcare", Sechenov First Moscow State Medical University, 119991 Moscow, Russia.

International Journal of Molecular Sciences
|January 20, 2021
PubMed

Insights

Adenovirus infection alters cell metabolism, leading to distinct cell death types like apoptosis and necrosis. Metabolomic analysis reveals these metabolic shifts precede cell death, aiding in oncolytic vector development.

Area of Science:

  • Virology
  • Cell Biology
  • Metabolomics

Background:

  • Viral infections, including adenovirus, cause significant intracellular metabolic fluctuations.
  • These metabolic shifts can trigger various forms of programmed cell death.
  • Understanding these metabolic changes is crucial for deciphering viral pathogenesis.

Purpose of the Study:

  • To review adenovirus-induced cell death mechanisms.
  • To correlate metabolic profile changes with specific cell death pathways.
  • To highlight the role of metabolomics in understanding adenovirus-mediated cell death.

Main Methods:

  • Review of experimental data on adenovirus-infected cells.
  • Analysis of metabolic profiles and their correlation with cell death.
  • Examination of specific metabolites involved in different cell death types.

Main Results:

  • Metabolome changes generally precede adenovirus-induced cell death.
  • Autophagic cell death linked to lactate, acetyl-CoA, and ATP release.
  • Apoptosis modulated by acetate and asparagine metabolism.
  • Necrotic cell death associated with ROS production and ATP depletion.
  • Ceramide accumulation contributes to all three cell death types.

Conclusions:

  • Metabolite analysis is key to understanding adenovirus-mediated cell death.
  • Characterizing metabolic shifts can inform the development of oncolytic adenoviral vectors.
  • Adenovirus-induced metabolic reprogramming offers therapeutic targets.
Keywords:
autophagymetabolitesviruses

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