Related Experiment Video

Updated: Nov 20, 2025

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

13.9K

MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10

Murong You1, Liang Zhang1, Xiaoxiang Zhang1

  • 1Department of Orthopedics, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi Province 330006, People's Republic of China.

Clinical Interventions in Aging
|January 20, 2021
PubMed
Abstract

Insights

Inhibition of miR-197-3p promotes osteoblast differentiation and combats osteoporosis by upregulating KLF10. This microRNA (miRNA) study reveals a novel mechanism for treating bone loss conditions.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Osteoporosis (OS) is a condition influenced by microRNA (miRNA) gene regulation.
  • miR-197-3p is notably dysregulated in osteoporosis patients.
  • This study investigates the specific role of miR-197-3p in osteoblast differentiation.

Purpose of the Study:

  • To elucidate the mechanism by which miR-197-3p regulates osteoblast differentiation.
  • To determine the relationship between miR-197-3p and Kruppel-like factor 10 (KLF10) in osteoporosis.
  • To evaluate the therapeutic potential of modulating miR-197-3p in osteoblast function.

Main Methods:

  • Ovariectomy was performed on rats to create a postmenopausal osteoporosis model.
  • Expression levels of miR-197-3p and KLF10 were quantified using qRT-PCR and Western blot.
  • In vitro assays included cell viability (MTT), ALP activity, and mineralization analysis in osteoblast cell lines.
  • Bioinformatics and luciferase reporter assays were employed to confirm the interaction between miR-197-3p and KLF10.

Main Results:

  • miR-197-3p expression was elevated in osteoporosis models, while KLF10 expression increased during osteoblast differentiation.
  • Inhibition of miR-197-3p significantly increased osteoblast differentiation markers (RUNX2, ALP, OCN, OSX), cell viability, and mineralization.
  • KLF10 was identified as a direct target of miR-197-3p, and its knockdown reversed the pro-osteogenic effects of miR-197-3p inhibition.

Conclusions:

  • Inhibition of miR-197-3p enhances osteoblast differentiation and mitigates osteoporosis.
  • Upregulation of KLF10 is a key mechanism through which miR-197-3p influences bone health.
  • Targeting miR-197-3p presents a potential therapeutic strategy for osteoporosis.
Keywords:
KLF10MC3T3-E1 cellsmiR-197-3posteogenic differentiationosteoporosis

More Related Videos

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders11:47

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

11.9K
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

4.8K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.2K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.5K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.5K