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Updated: Nov 20, 2025

Monitoring Protein Adsorption with Solid-state Nanopores

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

A whole area scanning-enabled direct-counting strategy for studying blocking efficiency in mitigating protein-solid

Haomin Liu¹, Yikun Huang², Yu Lei^3,4

¹Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT, 06269, USA.

Analytical and Bioanalytical Chemistry

|January 20, 2021

Summary

This summary is machine-generated.

A new whole area scanning (WAS) method directly counts fluorescent precipitates to assess protein blocking agents. This innovative technique enhances the evaluation of blocking agent efficiency in immunoassays.

Keywords:

Alkaline phosphatase Blocking efficiency Digital fluorescence counting Whole area scanning

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Monitoring Protein Adsorption with Solid-state Nanopores

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Counting Proteins in Single Cells with Addressable Droplet Microarrays

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Published on: July 6, 2018

Area of Science:

Biochemistry
Assay Development
Surface Science

Background:

Protein-solid surface interactions are critical for immunoassay performance.
Conventional methods for evaluating blocking agents have limitations.
New strategies are needed to accurately assess protein non-specific binding and blocking efficiency.

Purpose of the Study:

To develop and validate a novel whole area scanning (WAS)-enabled direct-counting strategy.
To evaluate the blocking efficiency of common blocking agents against streptavidin-alkaline phosphatase (Strep-ALP) non-specific binding.
To establish a sensitive method for investigating protein-surface interactions.

Main Methods:

Developed a WAS-enabled direct-counting strategy using fluorescent precipitates.
Utilized streptavidin-alkaline phosphatase conjugate (Strep-ALP) as a model protein.
Assessed blocking efficiency of BSA, casein, and dry milk by counting Strep-ALP precipitates after ELF™ 97 phosphate (ELFP) conversion to ELF™ 97 alcohol (ELFA).

Main Results:

The WAS strategy successfully enabled direct counting of individual fluorescent precipitates.
Quantified and compared the blocking efficiencies of BSA, casein, and dry milk.
Demonstrated the method's ability to mitigate non-specific binding of Strep-ALP.

Conclusions:

WAS-enabled direct counting provides a sensitive and novel approach for evaluating blocking agents.
This method offers a new perspective for studying protein-solid surface binding.
The developed strategy can significantly advance immunoassay development and optimization.