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Published on: April 18, 2014
TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology
1State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, South China Agricultural University, Guangzhou, China.
Abstract:
Efficient stacking of multiple genes is a critical element in metabolic engineering of complex pathways, synthetic biology, and genetic improvement of complex agronomic traits in plants. Here we present a high-efficiency multigene assembly and transformation vector system, TransGene Stacking II (TGS II), for these purposes. The operation process is described in detail, and the successful operation mainly depends on effective reagents, special Escherichia coli strains, and basic molecular biological means without other specific equipments.
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