Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

23.8K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
23.8K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.4K
4.4K
RNA-seq03:21

RNA-seq

11.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.1K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.8K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.8K
RNA Splicing01:32

RNA Splicing

59.0K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
59.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated targeted whole-genome and RNA-sequencing analysis of an intronic GNE variant in GNE myopathy.

Human genome variation·2026
Same author

Clonal Evolution of Endometriosis to Peritoneal Endometrioid Carcinoma via <i>PTEN</i> Biallelic Inactivation: A Case Report With Integrated Genomic and Pathological Profiling.

JCO precision oncology·2026
Same author

A Case of Multifocal Venous Malformation With Two Somatic Pathogenic Variants in the TEK Gene.

The Journal of dermatology·2025
Same author

A Case of Prolidase Deficiency With Long-Term Clinical Follow-Up.

The Journal of dermatology·2025
Same author

A Case of Pseudoxanthoma Elasticum Caused by a Novel Structural Variant of the ABCC6 Gene.

The Journal of dermatology·2025
Same author

Computational Comparison of Differential Splicing Tools for Targeted RNA Long-Amplicon Sequencing (rLAS).

International journal of molecular sciences·2025

Related Experiment Video

Updated: Nov 20, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.0K

Target-capture full-length double-strand cDNA sequencing for alternative splicing analysis.

Hiroki Ura1,2, Sumihito Togi1,2, Yo Niida1,2

  • 1Center for Clinical Genomics, Kanazawa Medical University Hospital, Ishikawa, Japan.

RNA Biology
|January 21, 2021
PubMed
Summary

The improved SMARTer Capture method enhances the detection of alternative splicing events by generating high-fidelity, full-length cDNA and using target-specific capture. This significantly increases accuracy and efficiency in characterizing gene expression.

Keywords:
Alternative splicingRNA-Sequencing (RNA-Seq)full-length double-strand cDNAnext-generation sequencingsplicing varianttarget-capture

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.0K
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.2K

Related Experiment Videos

Last Updated: Nov 20, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.0K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.0K
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.2K

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Alternative splicing generates diverse biological products from eukaryotic genes.
  • Defects in alternative splicing are linked to cellular dysfunction and disease.
  • Next-generation sequencing (NGS) detects splicing events, but standard RNA-Seq may not use full-length RNA.

Purpose of the Study:

  • To improve the SMARTer method for accurate detection of alternative splicing events.
  • To overcome limitations of non-specific genomic DNA amplification in the original SMARTer method.
  • To develop a target-capture method for enhanced full-length cDNA sequencing.

Main Methods:

  • Developed a target-capture full-length double-strand cDNA sequencing method (SMARTer Capture).
  • Generated high-fidelity, full-length cDNA using the SMARTer method.
  • Employed target-specific capture with exon probes for sequencing.

Main Results:

  • The SMARTer Capture method showed high correlation with the original SMARTer method's expression patterns.
  • Eliminated non-specific genomic DNA amplification, increasing the number and accuracy of detected splicing events.
  • Achieved 4-fold greater detection of alternative splicing events at the same read number compared to the original SMARTer method.

Conclusions:

  • The SMARTer Capture method is an improvement over the original SMARTer method.
  • It accurately characterizes alternative splicing repertories in targeted genes without biases.
  • Offers significantly increased efficiency in detecting splicing events.