Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.2K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.2K
Master Transcription Regulators02:23

Master Transcription Regulators

7.4K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.4K
Position-effect Variegation02:32

Position-effect Variegation

6.8K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.8K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.6K
3.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

24.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
24.6K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.0K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal Single-Cell RNA-Sequencing Reveals Evolution of Micro- and Macro-states in Chronic Myeloid Leukemia.

Cancer research·2026
Same author

Multiomic State-Transitions Reveal Post-Treatment Transcriptome Desynchronization in Acute Myeloid Leukemia.

bioRxiv : the preprint server for biology·2026
Same author

Overcoming vascular niche-mediated TKI resistance in acute myeloid leukemia through miR-126 inhibition.

NPJ systems biology and applications·2026
Same author

Inferring chromatin architecture at a single locus through probabilistic in situ DNA localization.

Nature communications·2026
Same author

Longitudinal single cell RNA-sequencing reveals evolution of micro- and macro-states in chronic myeloid leukemia.

bioRxiv : the preprint server for biology·2025
Same author

The proximal enhancer of the snail gene mediates negative autoregulatory feedback in Drosophila melanogaster.

Genetics·2025
Same journal

Expanding the C. elegans toolkit with gonad explants.

Development (Cambridge, England)·2026
Same journal

Nuclear Factor Y controls nutrient-adaptive epithelial growth by regulating mTOR in the Drosophila midgut.

Development (Cambridge, England)·2026
Same journal

Primordial germ cells differentially contribute to the germline in zebrafish.

Development (Cambridge, England)·2026
Same journal

Dissecting planar and vertical organiser signals in early chick neural development.

Development (Cambridge, England)·2026
Same journal

Real-time transcriptomic profiling of hPSC-derived cartilage during development identifies a key role for the extracellular matrix in homeostasis and protection.

Development (Cambridge, England)·2026
Same journal

In preprints - housekeeping the housekeeping genes.

Development (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Nov 20, 2025

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.7K

Dynamic patterning by morphogens illuminated by cis-regulatory studies.

Jihyun Irizarry1, Angelike Stathopoulos2

  • 1California Institute of Technology, Division of Biology and Biological Engineering, 1200 East California Blvd., Pasadena, CA 91125, USA.

Development (Cambridge, England)
|January 21, 2021
PubMed
Summary
This summary is machine-generated.

Understanding how Bicoid and Dorsal morphogens pattern Drosophila embryos is key. This study explores their spatiotemporal gene regulation and interactions with other factors during development.

Keywords:
ActivatorBicoid (Bcd)ChromatinCis-regulatory mechanismDorsal (Dl)Drosophila melanogasterEmbryonic patterningEnhancerGradientMorphogenRepressorThresholds

More Related Videos

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions
07:18

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

3.0K
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

8.0K

Related Experiment Videos

Last Updated: Nov 20, 2025

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.7K
Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions
07:18

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

3.0K
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

8.0K

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Morphogen gradients are crucial for embryonic development, but their interpretation by cells remains unclear.
  • Bicoid and Dorsal are key maternal transcription factors in Drosophila melanogaster development.
  • Understanding morphogen dynamics is essential for deciphering cell fate specification.

Purpose of the Study:

  • To investigate the spatiotemporal gene regulation by Bicoid and Dorsal morphogens in Drosophila embryos.
  • To elucidate how changing morphogen dynamics are interpreted by cells to specify developmental fates.
  • To explore the interplay of Bicoid and Dorsal with other regulatory inputs.

Main Methods:

  • Analysis of Bicoid and Dorsal transcription factor actions at enhancers.
  • Dissection of direct regulatory inputs on target genes.
  • Examination of spatiotemporal gene expression patterns.

Main Results:

  • Bicoid and Dorsal directly regulate target genes to pattern Drosophila embryos.
  • The actions of these factors at enhancers provide insights into morphogen dynamics.
  • Additional inputs modulate the spatiotemporal control of gene expression.

Conclusions:

  • The dynamic nature of morphogen concentration is critical for developmental patterning.
  • Bicoid and Dorsal play pivotal roles, but their function is context-dependent.
  • A comprehensive understanding requires considering multiple regulatory inputs acting in concert.