The phylogenetic analysis of Acrossocheilus paradoxus among the acrossocheilus base on complete mitochondrial DNA...

In our research, we used 17 sets of primers to amplify the complete mitochondrial DNA (mtDNA) of Acrossocheilus paradoxus. The total length of the mitochondrial genome is 16 597 bp and deposited in the GenBank with accession numbers KT751364. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNAs) and a major non-coding control region. Most genes were encoded on the H-strand, except for the ND6 and 8 tRNA genes, encoding on the L-strand. The nucleotide skewness for the coding strands of A. Paradoxus (GC-skew= -0.24, AT-skew = 0.07) is biased toward A and C. The negative GC-skew ranges from -0.35(ND6) to -0.21(CO1) and the AT-skew showed more positive varying from -0.23(ND4L) to 0.42(ND6). The phylogenetic analysis demonstrated that Acrossocheilus clustal together as one group.