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Updated: Nov 20, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of Eastern paradise fish (Polynemus dubius)
Jia Li1,2, Hui Yu1,2, Zhiqiang Ruan2
1BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China.
Abstract:
Eastern paradise fish, Polynemus dubius (also called P. longipectoralis), lives in rivers of the Southeastern Asia. Although its whole genome is not available, we first obtained its complete mitochondrial genome through next-generation sequencing. The length of its mitochondrial genome is 16 568 bp with 13 protein-coding genes, two rRNA genes, 22 tRNA genes and one control region. The distribution and arrangement of genes in the mitochondrial genome are similar to those of other vertebrates. The GC content is 43.08%, similar to the reported P. paradiseus. A phylogenetic tree was constructed to compare with nine related species by using MEGA 6.0. Through phylogenetic analysis, the relationship of species within the Polynemus genus is determined.
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