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Updated: Jun 5, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
The first near telomere-to-telomere genome assembly of Panulirus homarus homarus
Na Zhou1, Rujingwen Huan2, Hanxuan Wang1
1School of Pharmacy, Laboratory of Drug Discovery from Natural Resources and Industrialization, State Key Laboratory of Mechanism and Quality of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
Abstract:
The scalloped spiny lobster (Panulirus homarus homarus) is an economically important decapod crustacean with high aquaculture potential. Several chromosome-level genomes of this species have been reported. But the lobster or even entire shrimps did not have the telomere-to-telomere assembly until now. Therefore, we present the first near telomere-to-telomere genome assembly of P. h. homarus generated by using pure Oxford Nanopore Technologies ultra-long (ONT) reads and Hi-C sequencing. The final assembly anchored to 73 chromosomes with a contig N50 of 41.1 Mb. 73 chromosomes contain entire 146 telomeres, of which 51 chromosomes have no gaps. A total of 38,396 protein-coding genes were predicted. BUSCO analysis showed a completeness score of 99.8%, indicating a high degree of assembly completeness. This high-quality genomic dataset provides a valuable resource for comparative genomics, evolutionary studies, and genome-assisted breeding of spiny lobsters.
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