Related Experiment Video
Updated: Nov 20, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Complete mitochondrial genome sequence of the Indian clouded leopard (Neofelis nebulosa)
Wajeeda Tabasum1, Ara Sreenivas1, Kesav Kumar Bheemavarapu1
1Conservation Genetics Lab, LaCONES, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
Abstract:
The complete mitochondrial genome of sequence 16,859 bp of Indian clouded leopard (Neofelis nebulosa) has been sequenced using next generation sequencing technology Torrent PGM platform. The complete mitochondrial genome sequence of clouded leopard consists of 13 protein-coding, 22 tRNA, and two rRNA genes and a control region (CR). The mitochondrial genome is relatively similar to other felid mitochondrial genomes with respect to gene arrangement, composition, tRNA structures and skews of AT/GC bases to be typical of those reported for other mammals. The nucleotide composition of the genome shows that there is more A-T% than G-C% on the positive strand as revealed by positive AT and CG skews. The base composition of the mitochondrial genome of clouded leopard is as follows: A, 5362 bp (31.8%); C, 4560bp (27.0%); G, 2475 bp (14.6%); T, 4462 bp (26.4%). Most of the genes have ATG initiation codons, except ND1, ND2, ND3, ND4, ND6, and CYTB (ATA start codon).
Related Concept Videos
Animal Mitochondrial Genetics
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Export of Mitochondrial and Chloroplast Genes
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Evolutionary Relationships through Genome Comparisons

